rat anti-nidogen (ng2) Search Results


nidogen  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology nidogen
Nidogen, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-nidogen+(ng2)/Nidogen+Antibody/pmc03538028-113-45-46
Average 93 stars, based on 1 article reviews
nidogen - by Bioz Stars, 2026-11
93/100 stars
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rat anti-pecam-1  (Becton Dickinson)
90
Becton Dickinson rat anti-pecam-1
Hematoxylin/eosin staining shows teratomas derived from wild type (A) and mutant (B) ES cells that are composed of a variety of differentiated cells. (C–D) Perlecan immunofluorescent labeling in wild type (C) and mutant (D) teratomas. In wild type perlecan is expressed in the stroma (s), and in BMs surrounding vessels (arrow in C) and other structures including glands. Perlecan-null teratomas also have perlecan expression in the tumor stroma (s) and vessels. (E–F) Double fluorescent labeling with <t>PECAM-1</t> in red (E) and perlecan in green (F) reveal that the vasculature of the perlecan-null tumors is composed of a mixture of perlecan-positive (host-derived) and perlecan-negative (ES cell-derived; arrowheads) endothelial cells. (G) Quantification of perlecan-positive and perlecan-negative vessels per area in wild type (n = 10) and mutant teratomas (n = 10). Bars: (A–D) 250 µm; (E–F) 125 µm.
Rat Anti Pecam 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-nidogen+(ng2)/anti+cd31/pmc03540034-69-55-57
Average 90 stars, based on 1 article reviews
rat anti-pecam-1 - by Bioz Stars, 2026-11
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p histone h3 ser10  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc p histone h3 ser10
Hematoxylin/eosin staining shows teratomas derived from wild type (A) and mutant (B) ES cells that are composed of a variety of differentiated cells. (C–D) Perlecan immunofluorescent labeling in wild type (C) and mutant (D) teratomas. In wild type perlecan is expressed in the stroma (s), and in BMs surrounding vessels (arrow in C) and other structures including glands. Perlecan-null teratomas also have perlecan expression in the tumor stroma (s) and vessels. (E–F) Double fluorescent labeling with <t>PECAM-1</t> in red (E) and perlecan in green (F) reveal that the vasculature of the perlecan-null tumors is composed of a mixture of perlecan-positive (host-derived) and perlecan-negative (ES cell-derived; arrowheads) endothelial cells. (G) Quantification of perlecan-positive and perlecan-negative vessels per area in wild type (n = 10) and mutant teratomas (n = 10). Bars: (A–D) 250 µm; (E–F) 125 µm.
P Histone H3 Ser10, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti-nidogen+(ng2)/Phospho-Histone+H3+(Ser10)+Antibody/pmc03538028-113-26-29
Average 97 stars, based on 1 article reviews
p histone h3 ser10 - by Bioz Stars, 2026-11
97/100 stars
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Image Search Results


Hematoxylin/eosin staining shows teratomas derived from wild type (A) and mutant (B) ES cells that are composed of a variety of differentiated cells. (C–D) Perlecan immunofluorescent labeling in wild type (C) and mutant (D) teratomas. In wild type perlecan is expressed in the stroma (s), and in BMs surrounding vessels (arrow in C) and other structures including glands. Perlecan-null teratomas also have perlecan expression in the tumor stroma (s) and vessels. (E–F) Double fluorescent labeling with PECAM-1 in red (E) and perlecan in green (F) reveal that the vasculature of the perlecan-null tumors is composed of a mixture of perlecan-positive (host-derived) and perlecan-negative (ES cell-derived; arrowheads) endothelial cells. (G) Quantification of perlecan-positive and perlecan-negative vessels per area in wild type (n = 10) and mutant teratomas (n = 10). Bars: (A–D) 250 µm; (E–F) 125 µm.

Journal: PLoS ONE

Article Title: Perlecan Maintains Microvessel Integrity In Vivo and Modulates Their Formation In Vitro

doi: 10.1371/journal.pone.0053715

Figure Lengend Snippet: Hematoxylin/eosin staining shows teratomas derived from wild type (A) and mutant (B) ES cells that are composed of a variety of differentiated cells. (C–D) Perlecan immunofluorescent labeling in wild type (C) and mutant (D) teratomas. In wild type perlecan is expressed in the stroma (s), and in BMs surrounding vessels (arrow in C) and other structures including glands. Perlecan-null teratomas also have perlecan expression in the tumor stroma (s) and vessels. (E–F) Double fluorescent labeling with PECAM-1 in red (E) and perlecan in green (F) reveal that the vasculature of the perlecan-null tumors is composed of a mixture of perlecan-positive (host-derived) and perlecan-negative (ES cell-derived; arrowheads) endothelial cells. (G) Quantification of perlecan-positive and perlecan-negative vessels per area in wild type (n = 10) and mutant teratomas (n = 10). Bars: (A–D) 250 µm; (E–F) 125 µm.

Article Snippet: The following primary antibodies were used for immunohistochemistry: rabbit anti-laminin-1; rabbit anti-perlecan antibodies against domain V; rabbit anti-nidogen-1; rabbit anti-collagen IV; rabbit anti-collagen XVIII against endostatin (obtained from Dr. Rupert Timpl); rabbit anti-desmin (Sigma-Aldrich); rabbit anti-NG2 (Millipore); rabbit anti PDGFR (Cell Signaling); rat anti-flk-1 (obtained from Dr. Urban Deutsch, Bad Nauheim, Germany); rabbit anti-fibronectin (Chemicon); rat anti-PECAM-1 (Pharmingen) and mouse anti-smooth muscle cell a-actin (Sigma-Aldrich).

Techniques: Staining, Derivative Assay, Mutagenesis, Labeling, Expressing

EBs were generated from control (+/+) and perlecan mutant (−/−) ES cells. (A–F) The data are shown from PECAM-1 immunostainings of 5+12 days EBs cultured in 5% FCS-containing DMEM. A prominent vascular network is formed in wild type EBs (A), while mutants form endothelial networks that are less dense and smaller (B). (C–D) Treatment with 20 ng/ml VEGF 165 stimulates the formation of large PECAM-positive vascularized areas both in control and mutant EBs. (E–F) Treatment with 20 ng/ml FGF-2 strongly induces the formation of extensive vascular networks in both control and perlecan-deficient EBs. (G) Binding of FGFR1-AP to control (+/+) and perlecan mutant (−/−) 5+9 days EBs. The EBs were grown until confluency in 96-well tissue culture plates and fixed. The fixed EBs were incubated with increasing concentration of FGF-2 and exposed to FGFR1-AP at a concentration of 300 µg/ml. After washing to remove unbound receptor bound FGFR1-AP was measured with AP chromogenic substrate. The data are means of 8 EBs per data-point. Statistical differences were tested by Students t-test (***, p<0.001). The experiment was repeated 3 times. Bars: (A–F) 1 mm.

Journal: PLoS ONE

Article Title: Perlecan Maintains Microvessel Integrity In Vivo and Modulates Their Formation In Vitro

doi: 10.1371/journal.pone.0053715

Figure Lengend Snippet: EBs were generated from control (+/+) and perlecan mutant (−/−) ES cells. (A–F) The data are shown from PECAM-1 immunostainings of 5+12 days EBs cultured in 5% FCS-containing DMEM. A prominent vascular network is formed in wild type EBs (A), while mutants form endothelial networks that are less dense and smaller (B). (C–D) Treatment with 20 ng/ml VEGF 165 stimulates the formation of large PECAM-positive vascularized areas both in control and mutant EBs. (E–F) Treatment with 20 ng/ml FGF-2 strongly induces the formation of extensive vascular networks in both control and perlecan-deficient EBs. (G) Binding of FGFR1-AP to control (+/+) and perlecan mutant (−/−) 5+9 days EBs. The EBs were grown until confluency in 96-well tissue culture plates and fixed. The fixed EBs were incubated with increasing concentration of FGF-2 and exposed to FGFR1-AP at a concentration of 300 µg/ml. After washing to remove unbound receptor bound FGFR1-AP was measured with AP chromogenic substrate. The data are means of 8 EBs per data-point. Statistical differences were tested by Students t-test (***, p<0.001). The experiment was repeated 3 times. Bars: (A–F) 1 mm.

Article Snippet: The following primary antibodies were used for immunohistochemistry: rabbit anti-laminin-1; rabbit anti-perlecan antibodies against domain V; rabbit anti-nidogen-1; rabbit anti-collagen IV; rabbit anti-collagen XVIII against endostatin (obtained from Dr. Rupert Timpl); rabbit anti-desmin (Sigma-Aldrich); rabbit anti-NG2 (Millipore); rabbit anti PDGFR (Cell Signaling); rat anti-flk-1 (obtained from Dr. Urban Deutsch, Bad Nauheim, Germany); rabbit anti-fibronectin (Chemicon); rat anti-PECAM-1 (Pharmingen) and mouse anti-smooth muscle cell a-actin (Sigma-Aldrich).

Techniques: Generated, Mutagenesis, Cell Culture, Binding Assay, Incubation, Concentration Assay